Coding

Part:BBa_K2220025:Design

Designed by: Nan Rong   Group: iGEM17_BNU-China   (2017-10-22)


Secrete beta-tubulin-mGFP fusion protein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 286
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 542
    Illegal BamHI site found at 1325
    Illegal XhoI site found at 244
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2422
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2404
    Illegal BsaI.rc site found at 886
    Illegal BsaI.rc site found at 2288
    Illegal SapI site found at 1422


Design Notes

We have done the codon optimization.


Source

Beta-tubulin: http://www.ncbi.nlm.nih.gov/nucleotide/645912969 Alpha-factor comes from pyes2/ct/alpha-factor plasmid.


References